Research Paper Volume 16, Issue 15 pp 11501—11512

Figure 5. AL161431.1 activates TGF-β/SMAD Pathway to promote EMT and its cellular location. (A) qRT-PCR was used to measure the level of AL161431.1 in the nuclear and cytoplasmic of KMBC, QBC-939 and RBE cells. (B) FISH was performed to observe the cellular location of AL161431.1(red) and 18s(green) in RBE cells (magnification, ×400, scale bar, 20 μm and magnification, ×1000, scale bar, 10 μm). (C) Western blotting showed expression of different TGF-β/SMAD Pathway proteins and epithelial and mesenchymal after silencing AL161431.1 in QBC-939, and RBE cells. (D) Western blotting showed expression of epithelial and mesenchymal proteins after silencing AL161431.1 in QBC-939, and RBE cells.