Research Paper Volume 16, Issue 15 pp 11501—11512

Figure 2. Prediction of the functions of LncRNA AL161431.1 in CCA. (A) The protein interaction network of differential mRNA in the high- and low- AL161431.1 expression groups was constructed using Cytoscape, and the confidence cutoff was set to 0.4. (B) The most relevant 16 key protein nodes were selected using the tool MCODE within Cytoscape. (C, D) GO functional annotation and KEGG enrichment of genes were analyzed using GSEA. (E, F) Further visualization was performed to examine whether the gene sets of these functions and signaling pathways obtained in GSEA analysis were enriched on the left or right side of the abscissa according to the degree of differential expression (LogFC) from high to low. (G) GSVA analysis in the hall-markers gene set showed that the high-AL161431.1 expression of group was mainly enriched in metabolic and tumor-related pathways.