Research Paper Volume 16, Issue 11 pp 9727—9752

Figure 7. 14-3-3σ enhances the HR pathway activation. (A) The representative images of the RPA2 foci in AsPC-1 cells after 3 Gy carbon ion irradiation in different groups were analyzed by immunofluorescence (IF) staining. (B) Percentage of positive nuclei of RPA2 in AsPC-1 cells in different groups (5 foci or more per nucleus being considered positive). (C) The representative images of the RPA2 foci in MiaPaCa-2 cells after 3 Gy carbon ion irradiation in different groups were analyzed by IF staining. (D) Percentage of positive nuclei of RPA2 in MiaPaCa-2 cells in different groups (5 foci or more per nucleus being considered positive). (E) The representative images of the RAD51 foci in AsPC-1 cells after 3 Gy carbon ion irradiation in different groups were analyzed by IF staining. (F) Percentage of positive nuclei of RAD51 in AsPC-1 cells in different groups (5 foci or more per nucleus being considered positive). (G) The representative images of the RAD51 foci in MiaPaCa-2 cells after 3 Gy carbon ion irradiation in different groups were analyzed by IF staining. (H) Percentage of positive nuclei of RAD51 in MiaPaCa-2 cells in different groups (5 foci or more per nucleus being considered positive). (I) Relative protein expression of RAD51, pRPA2, RPA2 in si14-3-3σ and NC AsPC-1 cells, 0 h, 1 h, 4 h, 8 h, and 12 h post-irradiation. (J) Relative protein expression of RAD51, pRPA2, RPA2 in 14-3-3σ-overexpression and vector MiaPaCa-2 cells, 0 h, 1 h, 4 h, 8 h, and 12 h post-irradiation. For IF, at least 100 cells per condition were analyzed. Data were presented as the mean ± standard deviation (SD). *p <0.05, **p <0.01, ***p <0.001, ****p <0.0001, ns: not significant. Scale bar = 10 μm.