Research Paper Volume 16, Issue 11 pp 9727—9752

Figure 6. Silencing of 14-3-3σ causes the HRR defect after carbon ion radiation. (A–C) Gene Set Enrichment Analysis (GSEA) for the enriched gene sets in the 14-3-3σ high- and low-expression groups. (D) Bioinformatics analysis of the correlation between 14-3-3σ and genes related to homologous recombination (HR) and non-homologous end-joining (NHEJ) pathway. (E–H) The mRNA expression of ATM and ATR after the silencing or overexpressing of 14-3-3σ in AsPC-1 and MiaPaCa-2 cells. (I, J) Relative expression of pATM, ATM, pATR, ATR, pCHK2, CHK2, pCHK1, CHK1 in si14-3-3σ and NC AsPC-1 cells, 0 h, 1 h, 4 h, 8 h, and 12 h post-irradiation. (K, L) Relative expression of pATM, ATM, pATR, ATR, pCHK2, CHK2, pCHK1, CHK1 in 14-3-3σ-overexpression and vector MiaPaCa-2 cells, 0 h, 1 h, 4 h, 8 h, and 12 h post-irradiation. β-Actin was used as a loading control. Data were presented as the mean ± standard deviation (SD) (n=3); *p <0.05, **p <0.01, ***p <0.001, ****p <0.0001, ns: not significant.