Research Paper Volume 16, Issue 11 pp 9727—9752

Figure 3. 14-3-3σ overexpression confers carbon ion radioresistance of PAAD cells. The protein and mRNA levels of 14-3-3σ in AsPC-1 cells with 14-3-3σ overexpression plasmids in MiaPaCa-2 cells were detected by immunoblotting (A, B) and qPCR. (C). (D, E) Colony formation assays and survival fraction curves of MiaPaCa-2 cells after exposure to the indicated carbon ion irradiation dose (1 Gy, 2 Gy, and 3 Gy). (F) Cell proliferation of MiaPaCa-2 in different groups after 3 Gy carbon ion irradiation was determined by CCK-8 assay at 24 h, 48 h, and 72 h. (G, H) The proliferation of MiaPaCa-2 cells was detected by EdU assay. Scale bar = 100 μm. (I, J) The apoptosis rate of MiaPaCa-2 cells after 3 Gy carbon ion irradiation at 24 h in different groups. Scale bar = 10 μm. (K, L) The cell cycle distribution of MiaPaCa-2 cells after 3 Gy carbon ion irradiation at 6 h, 12 h, and 24 h in different groups. β-Actin was used as a loading control. Data were presented as the mean ± standard deviation (SD) (n=3); *p <0.05, **p <0.01, ***p <0.001, ****p <0.0001, ns: not significant.