Research Paper Volume 16, Issue 11 pp 9680—9691
Daphnoretin inhibited SCI-induced inflammation and activation of NF-κB pathway in spinal dorsal horn
Figure 3. Daphnoretin can inhibit SCI-induced inflammation and activation of the NF-κB pathway in spinal dorsal horn. (A) The mRNA expression of TNF-α and IL-1β in spinal dorsal horn was measured by PCR. (B) The protein expression of TNF-α and IL-1β in spinal dorsal horn was measured by ELISA. (C, D) The protein expression of p-NF-κB, NF-κB p-IκBα and IκBα was examined by Western blot. n = 4 /group. (E) Immunofluorescence showed the effect of Daphnoretin in spinal dorsal horn after SCI. DAPI (blue), p-NF-κB (red). **P < 0.01, ***P < 0.001****P < 0.0001 vs Sham group, ##P < 0.01, ####P < 0.0001 vs SCI group, the data were analyzed by one-way ANOVA with Tukey’s test.