Research Paper Volume 16, Issue 11 pp 9680—9691
Daphnoretin inhibited SCI-induced inflammation and activation of NF-κB pathway in spinal dorsal horn
Figure 2. Daphnoretin can inhibit SCI-induced glial cell activation in spinal dorsal horn. (A) Immunofluorescence showed the effect of Daphnoretin on glial cells in spinal dorsal horn after SCI. DAPI (blue), Iba1 (microglia green), GFAP (astrocytes, red). Scale bars: 100 μm. (B, C) The changes of fluorescence intensity (B) and number of positive cells (C) in each group were statistically analyzed. n = 3 mice/group, 2 sections/mice, ****P < 0.0001 vs Sham group, ###P < 0.001, ####P < 0.0001 vs SCI group, the data were analyzed by one-way ANOVA with Tukey’s test.