Research Paper Volume 16, Issue 11 pp 9625—9648

Figure 4. In vitro osteogenic performance assessment of zinc scaffold/BF Exo composite implant. (A) The CCK-8 experiment was used to measure the cell viability of BMSCs after osteogenic induction for 1, 3, 5, and 7 days; (B) The CCK-8 experiment was used to assess the cell viability of RAW264.7 cells after osteoclast induction for the same time periods; (C, D) ALP staining was conducted for BMSCs after 3 and 7 days of osteogenic induction, where darker staining indicated stronger positive expression; (E, F) Alizarin Red staining was performed for BMSCs after 14 and 21 days of osteogenic induction; (G) TRAP staining was conducted for RAW264.7 cells after 7 days of osteoclast induction (^*P < 0.05, ^**P < 0.01, ^****P < 0.0001, and ns for P > 0.05, n = 3).