Research Paper Volume 16, Issue 11 pp 9558—9568

Figure 7. Silencing of SIRT3 abolished the beneficial effects of orexin-A in preventing cellular senescence. Cells were transducted Ad-SIRT3 shRNA, followed by stimulation with IL-1β (10 ng/ml) with or without orexin-A (10 μM). (A) Successful knockdown of SIRT3; (B) Telomerase activity was measured; (C) Quantification of SA-β-gal staining; (D) The levels of Acetyl-p53; (E) The mRNA levels of p21 (^##P < 0.01 vs. vehicle group; ^{^^}P < 0.01 vs. IL-1β group; ^**P < 0.01 vs. IL-1β+ orexin-A group, n = 5).