Research Paper Volume 16, Issue 11 pp 9558—9568

Figure 2. Treatment with orexin-A inhibited IL-1β-induced expression of pro-inflammatory cytokines. Cells were stimulated with IL-1β (10 ng/ml) in the presence or absence of orexin-A (5 and 10 μM) for 12 hours. (A) mRNA of TNF-α and CXCL-1; (B) Protein levels of TNF-α and CXCL-1 (^##P < 0.01 vs. vehicle group; ^{^, ^^}P < 0.05, 0.01 vs. IL-1β group, n = 6).