Research Paper Volume 16, Issue 10 pp 9264—9279
Bufotalin enhances apoptosis and TMZ chemosensitivity of glioblastoma cells by promoting mitochondrial dysfunction via AKT signaling pathway
Figure 7. Bufotalin improved TMZ sensitivity of GBM cells in vitro and in vivo. (A–D) Cell apoptosis was measured by flow cytometry. U87 and U251 cells were treated with TMZ and bufotalin for 24 h. (E, F) CCK-8 assay was used to determine the cell viability of U87 and U251 cells treated with TMZ and bufotalin for 24 h. (G, H) Representative images and quantification of mouse tumors. (I) Schematic illustration of the molecular mechanism. Significant differences compared with the control are indicated by *p < 0.05, **p < 0.01, and ***p < 0.001.