Research Paper Volume 16, Issue 10 pp 9264—9279
Bufotalin enhances apoptosis and TMZ chemosensitivity of glioblastoma cells by promoting mitochondrial dysfunction via AKT signaling pathway
Figure 4. Bufotalin induced GBM cells apoptosis in vitro. (A–D) Cell apoptosis was measured by flow cytometry. U87 and U251 cells were treated with bufotalin for 24 h. (E–H) The changes in ΔΨm were monitored by JC-1 staining. (I, J) The expression levels of cleaved caspase-3 and caspase-3 were analyzed by western blot. Data are the mean ± SD of triplicate samples. The bar of the fluorescence image is shown in the figure. Significant differences compared with the control are indicated by *p < 0.05, **p < 0.01, and ***p < 0.001.