Research Paper Volume 16, Issue 11 pp 9547—9557
Neratinib stimulates senescence of mammary cancer cells by reducing the levels of SIRT1
Figure 9. Overexpression of SIRT1 abolished the effects of Neratinib in cellular senescence. Cells were transduced with Ad-SIRT1, followed by stimulation with Neratinib (5 μM). (A) Western blot results revealed successful overexpression of SIRT1; (B) Representative images of p53 (ac-K382) and p21; (C) The telomerase activity; (D) The SA-β-Gal assay was used to assess cellular senescence (^^, P<0.01 vs. vehicle group; ##, P<0.01 vs. Neratinib group).