Research Paper Volume 16, Issue 11 pp 9460—9469
High glucose enhances fibrosis in human annulus fibrosus cells by activating mTOR, PKCδ, and NF-κB signaling pathways
Figure 4. PKCδ is regulated in HG-promoted fibrotic protein expression in HAFCs. (A, B) HAFCs were treated with PKC inhibitor (GF109203x; 10 μM), PKCδ inhibitor (rottlerin; 10 μM) or transfected with PKCδ siRNA then applied with HG, and the indicated mRNA expression was examined by qPCR (n=5). (C) Cells were stimulated with HG, and the p-PKCδ expression was examined by Western blot (n=3). (D) The densitometry analysis of (C) was quantified. * p < 0.05 versus the control group. # p < 0.05 versus the HG-treated group.