Research Paper Volume 16, Issue 10 pp 9251—9263
Trimethylamine-N-oxide promotes osteoclast differentiation and oxidative stress by activating NF-κB pathway
Figure 1. Effects of TMAO and PDTC on the viability of RAW 264.7 cells. (A) Molecular structure of TMAO. (B) The cell viability of RAW 264.7 cells after treatment with different concentrations of TMAO for 12, 24, 48, and 72 h was evaluated by CCK-8. The cell viability of RAW 264.7 cells decreased significantly at 72 h with 200 μM TMAO treatment. (C) The cell viability of RAW 264.7 cells after treatment with different concentrations of PDTC for 2, 4, 8, and 12 h was evaluated by CCK-8. The cell viability of RAW 264.7 cells decreased significantly at 4 h with 50 μM PDTC treatment. * p < 0.05, ** p < 0.01.