Research Paper Volume 16, Issue 10 pp 9204—9215

Figure 2. Overexpression of DKK2 and the progression of oral squamous cell carcinoma. (A) Reverse transcription-quantitative polymerase chain reaction was used to verify the overexpression efficiency of the SCC15 cell line. (B) Western blot was used to detect the overexpression efficiency of SCC15 cell line. (C) After overexpression of DKK2, the proliferation of transfected SCC15 cell line was detected using EdU assay. (D) After overexpression of DKK2, the migration of cells was detected using scratch assay. (E) After overexpression of DKK2, the invasion of cells was detected using Transwell assay. ^*p < 0.05; ^**p < 0.01; ^***p < 0.001; ^****p < 0.0001.