Research Paper Volume 16, Issue 10 pp 9168—9187

Figure 5. Vitamin C and Chk1 inhibitor rescue neuron loss and synaptic impairments in mice exposed to chronic stress. (A) Representative immunoblots of Synapsin I, PSD95, GluA1, GluA2, β-actin in hippocampus (left) and cortex (right) of mice in different groups. (B) Quantification of the relative protein expression levels, which were normalized to the β-actin levels. n = 3 per group. (C) Representative images of Nissl staining in the CA1, CA3 and DG region of mice hippocampus. Scale bar: 50 μm (CA1 and CA3) or 100 μm (DG). (D) The quantitative analysis of the area occupied by neurons in the CA1, CA3 and DG region. n = 8-11 per group. All data represent mean ± SEM, *P<0.05, **P<0.01, ****P< 0.0001.