Research Paper Volume 16, Issue 10 pp 9168—9187

Figure 1. Acute and chronic stress induce DNA damage in animal models. (A) Representative immunoblots of γH2A.X and β-actin in hippocampal tissues from chronic unpredictable mild stress (CUMS), chronic restraint stress (CRS) model and control animals. (B) Quantification of the relative protein levels of γH2A.X, which were normalized to the β-actin levels. n = 3 per group. (C) Representative fluorescence images of γH2A.X (green), NeuN (red) and DAPI (blue) in hippocampus (CA1 and DG region) and cortex in CUMS rats. Scale bar: 50 μm. (D) Quantitative analysis of the fluorescence intensity of γH2A.X in C. n = 5 per group. (E) Representative fluorescence images of γH2A.X (green), NeuN(red) and DAPI (blue) in hippocampal CA1 region and cortex in acute restraint stress (ARS) mice. Scale bar: 50 μm. (F) Quantitative analysis of the fluorescence intensity of γH2A.X in E. n = 4 per group. Data are presented as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001.