Figure 8. PRKDC knockdown induced cell death, EMT, and halted the cell cycle in LIHC. (A, B) Relative cell death percentages in Huh-7 or Hep-3B cells using trypan blue staining. (C, D) Protein level of apoptosis related markers in Huh-7 or Hep-3B cells. (E, F) Protein level of EMT related markers in Huh-7 or Hep-3B cells. (G–J) Cell cycle analysis between PRKDC knockdown and negative control groups in Huh-7 or Hep-3B cells using flow cytometry.