Research Paper Volume 16, Issue 10 pp 8965—8979
CRYAB suppresses ferroptosis and promotes osteogenic differentiation of human bone marrow stem cells via binding and stabilizing FTH1
Figure 6. CRYAB regulates ferroptosis and osteogenic differentiation of BMSCs in an FTH1-dependent manner. (A) The protein levels of CRYAB and FTH1 were detected using a Western blotting assay (n=3). (B–D) Cellular Fe and ROS levels and cell viability were detected (n=3). (E) mRNA expression levels of OCN, OPN, RUNX2, and COLI were detected using qRT-PCR (n=3). (F) The activity of ALP was detected using an Alkaline Phosphatase Assay Kit (n=3). *: p < 0.05; **: p < 0.01; ***: p < 0.001; ****: p < 0.0001.