Research Paper Volume 16, Issue 10 pp 8965—8979
CRYAB suppresses ferroptosis and promotes osteogenic differentiation of human bone marrow stem cells via binding and stabilizing FTH1
Figure 5. Knockdown of FTH1 induces ferroptosis and suppresses osteogenic differentiation of BMSCs. (A) The efficiency of FTH1 knockdown was validated using Western blotting method (n=3). (B, C) Cellular Fe and ROS levels were detected after FTH1 knockdown in BMSCs (n=3). (D, E) The cell viabilities were detected using CCK-8 assay (n=3). (F) mRNA expression levels of OCN, OPN, RUNX2, and COLI when FTH1 was silenced were detected using qRT-PCR (n=3). (G) The activity of ALP was detected using an Alkaline Phosphatase Assay Kit (n=3). ns: no significance; *: p < 0.05; **: p < 0.01; ***: p < 0.001; ****: p < 0.0001.