Research Paper Volume 16, Issue 10 pp 8965—8979
CRYAB suppresses ferroptosis and promotes osteogenic differentiation of human bone marrow stem cells via binding and stabilizing FTH1
Figure 4. CRYAB regulates the lactylation of FTH1 in BMSCs. (A) Protein level of FTH1 was assessed after CRYAB knockdown and MG132/BafA1 treatment using Western blotting assay (n=3). (B, C) Protein level of FTH1 was detected after 2-DG or oxamate treatment (n=3). (D) Protein level of FTH1 was detected after 2-DG treatment (20 mM) and sodium lactate treatment (Nala, 20 mM) or CRYAB overexpression by Western blotting method (n=3).