Research Paper Volume 16, Issue 10 pp 8965—8979
CRYAB suppresses ferroptosis and promotes osteogenic differentiation of human bone marrow stem cells via binding and stabilizing FTH1
Figure 3. CRYAB interacts with FTH1 and regulates the ferroptosis of BMSCs. (A, B) Co-IP assay was used to detect the interaction between CRYAB and FTH1 (n=3). (C, D) Cellular Fe and ROS levels were detected after CRYAB knockdown in BMSCs (n=3). (E, F) The protein and mRNA levels of FTH1 were detected after CRYAB knockdown (n=3). (G) The cell viabilities were detected using CCK-8 assay (n=3). ns: no significance; *: p < 0.05; **: p < 0.01; ***: p < 0.001.