Research Paper Volume 16, Issue 10 pp 8965—8979
CRYAB suppresses ferroptosis and promotes osteogenic differentiation of human bone marrow stem cells via binding and stabilizing FTH1
Figure 1. CRYAB positively regulates the osteogenic differentiation of BMSCs. (A) mRNA expression level of CRYAB in osteoporosis samples was detected using qRT-PCR. (B, C) mRNA expression levels of CRYAB, OCN, OPN, RUNX2, and COLI when CRYAB was overexpressed or silenced were detected using qRT-PCR (n=3). (D, E) Protein expression levels of CRYAB, OCN, OPN, RUNX2, and COLI when CRYAB was overexpressed or silenced were detected using the Western blotting method (n=3). (F) The activity of ALP was detected using an Alkaline Phosphatase Assay Kit (n=3). *: p < 0.05; **: p < 0.01; ***: p < 0.001; ****: p < 0.0001.