Research Paper Volume 16, Issue 10 pp 8898—8921

Figure 9. Knockdown of CUL2 inhibits cell proliferation and invasion in HCCLM3 cells. (A) The knockdown efficiency of shCUL2 was examined in HCCLM3 cells with western blotting, and qRT-PCR (B) Cellular growth curves were evaluated by CCK-8 assays in HCCLM3 cells. (C) Representative images and quantification of colony formation assays of HCCLM3 transfected with shCUL2. (D) Representative images and quantification of EdU assays to evaluate cell proliferation ability after transfecting shCUL2, magnification, ×200; scale bars, 50 μm. (E) Representative images and quantification of transwell assay to examine the invasion ability, magnification, ×200; scale bars, 100 μm. *p < 0.05, ** p < 0.01, *** p < 0.001, ns, no significance.