Research Paper Volume 16, Issue 10 pp 8880—8897

Figure 5. Application of PD-1 antibody to substantiate PDIA3’s role in endorsing M2 polarization of tumor-associated macrophages and prompting tissue protease secretion, mediated by STAT3/PD-1 signaling modulation. (A) Protein levels of pSTAT3 and PD-1 in cells with PDIA3 overexpression versus or-NC control, and PD-1 inhibitor versus PD-1 NC treatment assessed by western blot (n=3 per group). Statistical evaluation via T-test,* *P < 0.01 versus corresponding control. (B) Levels of XBP-1, arginase-1, cathepsin-L, cathepsin-K, and IL-6 proteins in THP-1 cells under differing conditions of PDIA3 expression and PD-1 inhibition, determined via western blot (n=3 per group). Statistical evaluation via T-test,* *P < 0.01 versus corresponding control.