Research Paper Volume 16, Issue 10 pp 8880—8897
PDIA3 driven STAT3/PD-1 signaling promotes M2 TAM polarization and aggravates colorectal cancer progression
Figure 4. PDIA3 facilitates M2 polarization of tumor-associated macrophages and augments tissue protease secretion through modulation of STAT3/PD-1 signaling. (A) Comparison of p-STAT3 and PD-1 protein levels in cells with PDIA3 overexpression versus or-NC control, and PDIA3 knockdown versus sh-NC control, as determined by western blot (n=3 per group). Statistical evaluation via Ttest,* *P < 0.01 versus corresponding control. (B) Protein expression of XBP-1, arginase-1, cathepsin-L, cathepsin-K, and IL-6 in PDIA3-altered THP-1 cells, assessed by western blot (n=3 per group). Statistical evaluation via T-test,* *P < 0.01 versus corresponding control.