Figure 10. In vitro and in vivo phase separation results of RELA. (A) Predicted phase separation ability of the RELA protein’s IDR region. (B, C) In vitro liquid-liquid phase separation (LLPS) of EGFP-tagged RELA under various physicochemical conditions. (D) In vitro fluorescence recovery after photobleaching (FRAP) of EGFP-RELA. (E) In vivo droplet fusion events of EGFP-RELA. (F) Co-localization of RELA with specific transcription elements, suggesting intricate relationships in gene expression regulation.