Research Paper Volume 16, Issue 10 pp 8732—8746

Figure 5. miR-101a-3p mimic inhibits the apoptosis of Neuro-2a cells induced by MPP⁺. To confirm the role of miR-101a-3p in Neuro-2a cells treated with MPP⁺, miR-101a-3p was overexpressed in Neuro-2a cells by transfecting miR-101a-3p mimics before Neuro-2a cells were exposed to MPP⁺. There were 4 groups: control group, MPTP group, MPP⁺+miR-control group, and MPP⁺+miR-101a-3p mimic group. (A) Detection via qRT-PCR of miR-101a-3p expression in each group of cells. (B) Detection of ROCK2 mRNA expression in each group of cells via qRT-PCR. (C) Western blot detection of ROCK2 protein expression level in each group of cells. (D) Western blot was utilized to detect α-synuclein protein expression in each group of cells. (E) Flow cytometry was utilized to detect the apoptosis level of Neuro-2a cells in each group. ^**P < 0.01 and ^***P < 0.001.