Research Paper Volume 16, Issue 10 pp 8732—8746
miR-101a-3p/ROCK2 axis regulates neuronal injury in Parkinson’s disease models
Figure 4. MPP+ suppresses miR-101a-3p expression and induces the apoptosis of Neuro-2a cells. (A) Western blotting was utilized to detect α-synuclein protein expression in control group and MPP+ group. (B) Detection of miR-101a-3p expression in Neuro-2a cells of the control group and MPP+ group by qRT-PCR. (C) Flow cytometry was utilized to detect the apoptosis level in control group and MPP+ group. (D) Detection of ROCK2 mRNA expression level in Neuro-2a cells in the control group and MPP+ group by qRT-PCR. (E) Western blot detection of ROCK2 protein expression level in Neuro-2a cells of control group and MPP+ group. ***P < 0.001.