Research Paper Volume 16, Issue 10 pp 8645—8656

Figure 4. Anti-inflammatory effect of mangiferin on LPS-exposed cells. The concentrations of IL-1β, IL-6, and TNF-a in mangiferin-treated culture medium are measured by ELISA assay. Data are presented as mean ± SD. One-way ANOVA is used for statistical analysis. (A) Concentrations of IL-1β in control, LPS, 20 μmol/L mangiferin with LPS, and 40 μmol/L mangiferin with LPS are 23.23 pg/mL, 62.11 pg/mL, 49.37 pg/mL, and 35.64 pg/mL, respectively, all ^***P < 0.001. (B) Concentrations of IL-6 in control, LPS, 20 μmol/L mangiferin with LPS, 40 μmol/L mangiferin with LPS are 39.52 pg/ml, 120.70 pg/mL, 71.41 pg/mL, and 52.57 pg/mL, respectively. ^**P = 0.0066 for LPS vs. 20 μmol/L mangiferin with LPS, P = 0.23 for 20 μmol/L mangiferin with LPS vs. 40 μmol/L mangiferin with LPS, no significance is observed. All other comparisons are presented as ^***P < 0.001. (C) Concentrations of TNF-a in control, LPS, 20 μmol/L mangiferin with LPS, and 40 μmol/L mangiferin with LPS are 18.61 pg/mL, 86.98 pg/mL, 75.60 pg/mL, and 45.80 pg/mL, respectively, all ^***P < 0.001.