Research Paper Volume 16, Issue 10 pp 8630—8644

Figure 5. Neferine activated Nrf2/HO-1 and inhibited TGF-β/p-Smad2/3 signaling pathway. (A) Western blotting was performed to assess the expression of Nrf2 and HO-1 in the atrium, and the statistical results are shown on the right (n = 3). (B) Protein expression of TGF-β, p-Smad2/3, Smad2, and Smad3 in the atria was determined by western blot, with the corresponding statistical results on the right (n = 3). (C) HL-1 cells were treated with 2, 5, and 10 μM neferine for 24 hours, and Nrf2 and HO-1 protein expression were assessed by western blot, with the statistical results shown on the right (n = 3). (D) HL-1 cells were treated with 100 nM Ang II and 10 μM neferine for 24 hours, and Nrf2 and HO-1 protein expression were determined by western blot, with the statistical results shown on the right (n = 3). GAPDH served as an internal control. Data are presented as mean ± SEM, and n represents the number of samples. *P < 0.05 for the difference compared with the Saline group; ^#P < 0.05 for the difference compared with the Ang II group.