Research Paper Volume 16, Issue 10 pp 8599—8610

Figure 4. Effect of ibuprofen on DNA strand break and DNA repair (γ-H2AX⁺ foci) in human skeletal muscle after HIIE. DNA strand break in nuclei (blue) is visualized by TUNEL immunofluorescence stains (green light) (A). DNA strand break at 3 h and 24 h after HIIE was comparable to pre-exercise baseline during both placebo and ibuprofen trials (B). γ-H2AX⁺ foci, shown in representative immunohistochemical stains (brown precipitate), are located in both myonuclei and the nuclei outside myofibers (C). γ-H2AX⁺ nuclei at 3 h and 24 h after HIIE was similar to pre-exercise baseline during both placebo and ibuprofen trials (D). Abbreviations: HIIE: high intensity interval exercise; TUNEL: terminal deoxynucleotidyl transferase dUTP nick end labeling.