Research Paper Volume 16, Issue 10 pp 8585—8598

Figure 4. In vitro, ZNF516 inhibits trophoblast migration, invasion, and viability. (A) RT-qPCR was used to measure how much ZNF516 was expressed in HTR8/SVneo cells after they were treated with oe-ZNF516 and anti-miR. (B) Western blotting was used to measure the level of ZNF516 in HTR-8/SVneo cells that had been treated with oe-ZNF516 and anti-miR-371-5p. (C) ZNF516 reduced the viability of HTR48/SVneo cells. The vitality of HTR 8/SVneo cells was assessed using CCK-8 assays after transfecting them with oe-ZNF516, anti-miR, or empty vectors for durations of 1, 2, 3, 4, or 5 days. (D) The proliferation potential of HTR8/SVneo cells was evaluated through the use of a colony formation test. The growth of HTR8/SVneo cell colonies was reduced by ZNF516 (scale bar = 2 mm). (E) Colonies were quantified using ImageJ software. (F, G) We used wound healing experiments to look at how overexpressing ZNF516 affected the ability of HTR48/SVneo cells to move. HTR48/SVneo cells transfected with oe-ZNF516, anti-miR, or empty vectors were seeded in six-well plates 48 hours posttransfection. Images were captured at 0, 24, and 48 hours. Scratched wound boundaries are marked by black lines (scale bar = 200 μm). (H, I) HTR8/SVneo cell migration and invasion were measured using Transwell assays (scale bar = 50 μm). N = 3; all results are presented as means ± SEMs. ^*P < 0.05, ^**P < 0.01, and ^***P < 0.001, respectively, in comparison to the vector + control group. ZNF516, overexpressed-ZNF516; anti-miR, miR-371-5p inhibitors.