Research Paper Volume 16, Issue 10 pp 8585—8598

Figure 2. ZNF516 expression in the placentas of individuals with PE and those with pregnancies without abnormalities. (A) This study employed the RT-qPCR technique to evaluate the mRNA expression levels of ZNF516 in the placenta of both the EOPE and LOPE groups. Control, n = 71; EOPE, n = 34; and LOPE, n = 55. ^****P < 0.0001. (B) Western blotting was used to assess the protein expression of ZNF516 in placental tissue. n = 3. (C, D) Volcano plot and heatmap showing the DEGs in the GSE96985 dataset. n = 308, P < 0.05, red arrows, ZNF516. (E) KEGG pathway analysis of the biological processes associated with the upregulated DEGs. (F) The DEGs primarily exhibit enhanced GO keywords related to biological processes, cellular components, and molecular functions. (G, H) Through the utilization of cytoHubba, the PPI network was utilized to choose the top 30 hub genes.