Research Paper Volume 16, Issue 9 pp 8320—8335
Intracranial aneurysm circulating exosome-derived LncRNA ATP1A1-AS1 promotes smooth muscle cells phenotype switching and apoptosis
Figure 7. Knockdown of ATP1A1-AS1 inhibits VSMC phenotype switching and apoptosis. (A) ATP1A1-AS1 siRNA was used to knockdown ATP1A1-AS1 in VSMCs. The expression level of ATP1A1-AS1 was determined using qRT-PCR. (B, C) WB was performed to detect contraction markers proteins (B) and MMP-2 and MMP-9 (C) expression levels after knockdown of ATP1A1-AS1 in pathological VSMCs, simulated using 400 μM hydrogen peroxide. (D, E) Quantification of protein expression levels. (F) TUNEL assay used to detect apoptosis after knockdown of ATP1A1-AS1 in pathological VSMCs (*p < 0.05, **p < 0.01, ***p < 0.001).