Research Paper Volume 16, Issue 9 pp 8320—8335

Figure 6. Overexpression of ATP1A1-AS1 promotes VSMC phenotype switching and apoptosis. (A) ATP1A1-AS1 mimics were used to overexpress ATP1A1-AS1 in VSMCs. The quantification of ATP1A1-AS1 expression was performed using qRT-PCR. (B, C) WB were performed to quantify the protein expression levels of contractile markers (B) and MMP-2 and MMP-9 (C) after overexpression of ATP1A1-AS1 in VSMCs. (D, E) WB was performed to detect contraction markers (D) and MMP-2 and MMP-9. (E) Protein expression levels after overexpression of ATP1A1-AS1 in pathological VSMCs, simulated using 400 μM hydrogen peroxide. (F–I) Quantification of protein expression levels. (J) TUNEL assay used to detect apoptosis after overexpression of ATP1A1-AS1 in pathological VSMCs. (^*p < 0.05, ^**p < 0.01, ^***p < 0.001).