Research Paper Volume 16, Issue 10 pp 8524—8540

Figure 9. The effects of the KCNQ1OT1/SNHG14/XIST-miR-873-5p-HAMP axis on H460 cell proliferation, invasion and migration. The miR-873-5p mimics and the HAMP, KCNQ1OT1, SNHG14, and XIST overexpression plasmids were transfected into H460 cells. (A, B) The HAMP profile was evaluated using RT-PCR and western blot analyses. (C) Cell proliferation was assessed via a CCK-8 assay. (D) The ability of cells to form colonies was evaluated through a cell colony formation assay. (E) Cell proliferation was examined via an EdU staining assay. The scale bar is 50 μm. (F) Transwell assays revealed the migratory and invasive traits of H460 and PC9 cells. The scale bar is 200 μm. (G) p-NF-κB p65 protein levels in whole cells and in the nucleus were assessed through western blot analysis. ^**P < 0.01, ^***P < 0.001 vs. vector. ^&&P < 0.01, ^&&&P < 0.001, vs. HAMP; ^#, ^##, and ^### indicate P < 0.05, P < 0.01, and P < 0.001 vs. HAMP+miR-873-5p. n = 3.