Research Paper Volume 16, Issue 10 pp 8524—8540

Figure 8. Regulatory mechanism of the lncRNA KCNQ1OT1/SNHG14/XIST-miR-873-5p-HAMP axis. (A) miR-873-5p targets the 3’UTR of HAMP mRNA, as determined via TargetScan. (B) The lncRNAs XIST, KCNQ1OT1, and SNHG14 potentially target miR-873-5p. (C, D) A dual-luciferase reporter assay revealed the interaction of miR-873-5p-HAMP, miR-873-5p-KCNQ1OT1, miR-873-5p-SNHG14, and miR-873-5p-XIST in 293T cells. (E) The interaction between miR-873-5p and KCNQ1OT1/SNHG14/XIST in 293T cell lysates was verified through pull-down analysis. (F) The expression levels of miR-873-5p were investigated following the transfection of KCNQ1OT1, SNHG14, and XIST overexpression plasmids into H460 cells. (G, H) H460 cells were transfected with HAMP overexpression plasmids, miR-873-5p mimics, or KCNQ1OT1, SNHG14, or XIST overexpression plasmids. Subsequently, HAMP mRNA and protein levels were evaluated. ^***P < 0.001 vs. vector, ^&&&P < 0.001 vs. miR-873-5p. n = 3.