Research Paper Volume 16, Issue 10 pp 8524—8540

Figure 7. HAMP upregulated the NF-κB pathway in NSCLC cells. (A) JSH-23 (10 μM) was used for treating H460 with HAMP overexpression, and western blot analysis was carried out to evaluate p-NF-κB p65 protein levels in whole cells and in the nucleus. (B) Cell proliferation was assessed using a CCK-8 assay. (C) The ability of cells to form colonies was evaluated through a cell colony formation assay. (D) Cell proliferation was examined using an EdU staining assay. The scale bar is 50 μm. (E) Transwell assays were employed to evaluate the migratory and invasive properties of H460 cells. Scale bar = 200 μm. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001 vs. vector. ^#P < 0.05, ^##P < 0.01, ^###P < 0.001 vs. HAMP. n = 3.