Research Paper Volume 16, Issue 10 pp 8524—8540

Figure 3. The effects of HAMP knockdown on the proliferation and invasion of NSCLC cells. (A) A cellular model for HAMP knockdown was established in H460 and PC9 cells, and western blot analysis was conducted to assess HAMP protein levels. (B) Cell proliferation was evaluated through a CCK-8 assay. (C) The ability of cells to form colonies was determined using a cell colony formation assay. (D) An EdU staining assay was used to evaluate cell proliferation. The scale bar is 50 μm. (E, F) Transwell assays revealed the migratory and invasive attributes of H460 and PC9 cells. Scale bar = 200 μm. NS P > 0.05, ^*P < 0.05, ^**P < 0.01, ^***P < 0.001 vs. sh-NC (short hairpin RNA negative control). n = 3.