Figure 2. Effect of aging on adipose tissue fibrosis and adipocyte size distribution. (A, B) Masson’s Trichrome staining of adipose tissue sections (A) were quantified for staining intensity using ImageJ and plotted (B). (C, D) Adipose tissue sections from different mice groups were stained with H&E stain (C) and adipocytes size was measured using ImageJ software (D). (n = 3–5 mice per group) P-value < 0.05 = *, ns = non-significant.