Research Paper Volume 16, Issue 10 pp 8511—8523
LncRNA SH3BP5-AS1 promotes hepatocellular carcinoma progression by sponging miR-6838-5p and activation of PTPN4
Figure 6. HIF-1α regulated SH3BP5-AS1 transcription by directly binding to its promoter. (A) The mRNA level of SH3BP5-AS1 in the hypoxic HCC cells was measured using a qRT-PCR experiment. (B) HIF-1α binding motif. (C) Possible HIF-1α binding location in the SH3BP5-AS1 promoter. (D) CHIP-qPCR assay. (E) Dual luciferase reporter assay in the HCC cells. (F) qRT-PCR experiment in the HCC cells.