Research Paper Volume 16, Issue 10 pp 8511—8523

Figure 3. PTPN4 was a functional target of miR-6838-5p. (A) The PTPN4 mRNA 3-UTR’s miR-6838-5p binding location. (B, C) MiR-6838-5p prevented PTPN4’s mRNA and protein from being expressed. (D) Luciferase activity was significantly decreased when PTPN4-WT and miR-6838-5p were co-transfected. (E) The qRT-PCR technique was used to measure the mRNA level of PTPN4 in the HCC cells. (F, G) PTPN4 silencing prevented the miR-6838-5p inhibitor’s promotion effect on HCC cells’ growth. (H, I) The cell scratch and transwell assays were used to identify the migration and invasion capacity of HCC cells.