Research Paper Volume 16, Issue 9 pp 8142—8154
4-hydroxysesamin protects rat with right ventricular failure due to pulmonary hypertension by inhibiting JNK/p38 MAPK signaling
Figure 5. Protective effect of 4-HS on right ventricular tissue in PH rats. (A) TUNEL staining to detect the effect of 4-HS pretreatment on apoptosis in right ventricular myocardial tissue (Scale bar, 50 μm). (B) Sirius scarlet staining to detect the effect of 4-HS pretreatment on right ventricular myocardial tissue (Scale bar, 50 μm). Effect of fibrosis. (C) ELISA assay to detect changes in the expression of inflammatory factors IL-1β, IL-6, IL-10, and TNF-α in the serum of rats in each group of the experiment. (D) Western blot assay to detect changes in the expression of JNK, p-JNK, p38 MAPK, and p-p38 MAPK proteins in the tissues of pulmonary arteries in rats. (E) Western blot assay to detect changes in the expression of CDK2, CyclinD1, and PCNA proteins in the tissues of pulmonary arteries in rats.