Research Paper Volume 16, Issue 10 pp 8484—8496
Shexiang tongxin dropping pill alleviates myocardial injury induced by coronary microembolization by down-regulating APOC1 to inhibit STAT3 signaling pathway
Figure 4. STDP regulates APOC1 expression to attenuate LPS-induced inflammation and apoptosis in cardiomyocytes. Note: (A) MTT assay detected the viability of rat primary cardiomyocytes; (B) AnnexinV/PI staining and flow cytometry analyze the cell apoptosis of cardiomyocytes; (C) Western blot detected the expressions of Cleaved caspase-3, BAX and Bcl-2; (D, E) qRT-PCR detected the expressions of APOC1, IL-6, IL-1β and TNF-α in LPS-induced cardiomyocytes; (F) Western blot detected the expressions of STAT3 signal pathway related proteins. *P < 0.05, ** P < 0.01. Each experiment was repeated for 3 times. STDP, Shexiang Tongxin dropping pill; LPS, lipopolysaccharide.