Research Paper Volume 16, Issue 9 pp 7870—7888
Leptin attenuates the osteogenic induction potential of BMP9 by increasing β-catenin malonylation modification via Sirt5 down-regulation
Figure 8. The effects of Sirt5 on BMP9-induced osteogenic markers in C3H10T1/2 cells. (A, B) qPCR and Western blot assay show the effect of knocking down Sirt5 on Runx2. (C) Quantification of the Western blot assay. (D, E) qPCR and Western blot assay show the effect of knocking down Sirt5 on OPN. (F) Quantification of the Western blot assay. (G) ALP staining shows the effect of knocking down Sirt5 on ALP activity. (H) Quantitative results of ALP staining. (“*” P < 0.5, “**” P< 0.01; “#” P<0.05, “##” P < 0.01).