Research Paper Volume 16, Issue 9 pp 7523—7534

Figure 1. WEW decreases cellular senescence in human fibroblasts and cancer cells. Cells were treated with WEW and induced to senesce with H₂O₂. (A) Images of morphological changes during oxidative stress induction of senescence in BJ and T47D cells. Both cell types adopted a flattened and enlarged morphology. (B) SA-β-gal staining of senescent fibroblasts and cancer cells treated and non-treated with WEW. Images were taken using an inverted phase contrast microscope after SA-β-gal activity was detected by incubating the fixed cells in SA- β -gal solution at 37° C. (C) SA- β -gal activity was assayed by manually scoring SA-β-gal positive cells. Results are expressed as mean values (+SEM) of cell count in one of three independent experiments. Significance was calculated by a two-tailed Student’s t test. *P < 0.05, **P < 0.01, ***P < 0.001