Research Paper Volume 16, Issue 8 pp 6673—6693

Figure 6. Unbiased quantitative proteome profile of the SASP from human corneal endothelial cells. (A) Conditioned media (CM) from UV-A-induced human senescent corneal endothelial cells (hCEnCs) (n = 4), IR-induced senescent hCEnCs (n = 4) and non-senescent hCEnCs (control) (n = 4) were fractionated using HPLC and analyzed using mass spectrometry. We show the principal component score analysis of CM from senescent and non-senescent hCEnCs. (B) Venn diagram showing unique and significantly up-regulated (left) and down-regulated (right) SASP proteins (≥ 2 unique peptides, fold change ≥ 1.5, q-value ≤ 0.05) in UV-A- and IR-induced senescent hCEnCs. (C, D) Volcano plot showing Q-values (−log10) vs. fold change of (log2) UV-A-induced senescent and control hCEnCs (C) as well as IR-induced senescent and control hCEnCs (D). Blue, downregulated proteins; red, upregulated proteins; and gray, no significant change (≥ 2 unique peptides, fold change ≥ 1.5, q-value ≤ 0.05). (E) Pathway and network analysis of secreted proteins that are significantly increased in the SASP from UV-A-induced senescent hCEnCs. (F) Pathway and network analysis of secreted proteins that are significantly increased in the SASP from IR-induced senescent hCEnCs.