Research Paper Volume 16, Issue 8 pp 6673—6693

Figure 1. Morphological changes of human corneal endothelial cells after UV-A exposition. (A) Representative images of human corneal endothelial cells 1 (top), 5 (middle-upper) and 10 days (middle-lower: low magnification) (bottom: high magnification) after UV-A or IR treatment. Cells were either mock-treated or treated with four doses of UV-A (2.5, 5, 10, and 20 J/cm2) or IR (10 Gy). Scale bar: 100μm. (B) SA-β-Gal staining (left panels) and EdU labeling (right panels) of human corneal endothelial cells (hCEnCs). Cells were treated or not with 5 J/cm2 UV-A or 10 Gy IR, cultured for 10 days, and then SA-β-Gal staining and EdU labeling performed. Representative images are shown. Scale bar: 100μm. (C) quantification of SA-β-Gal staining, and (D) quantification of EdU labeling, plotted as mean of % of positive cells from five independent experiments.